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The DNA was extracted and purified from the sample using the DNeasy PowerSoil Pro Kit (Qiagen Italia, Milan, Italy)

So just to recap first, allulose is a natural sugar that stimulates GLP-1, similar to, but a lot more modestly than, modern weight loss meds

50 mg sits near the low end of the typical range, often used early in titration rather than as a final target

There is some research to show vegetarians and/or vegans may not be getting enough B12 and should look to take a supplement if levels are low (chat to Pharmacist or GP)