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TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions

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found that reduced levels of PUFA-PLs could promote resistance to ferroptosis, while decreased PUFA-PLs in neutrophils could enhance ferroptosis susceptibility, indicating that PUFA-PLs are the primary factors determining ferroptosis sensitivity in lymphocytes and myeloid cells (123)

The method of any one of embodiments 118-138, wherein the composition is free or substantially free of water, C1-C6 alcohols or C1-C6 glycols, C1-C4 alcohols or C1-C4 glycols, or any combination thereof