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In the reaction mixtures without enzyme, varying amounts of MBB (0-300 l of 1 mM stock solution which gave final concentration 0-100 M) were added into different fluorescent cuvettes already containing 3.0 ml of phosphate buffer (pH 6.5) and 100 l of GSH stock solution (3 mM)

Although earlier research suggested that NO imposes its effect via direct interaction with the cytochrome bc1 complex (submitochondrial particles found within CIII) (Poderoso et al., 1996