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Pyocyanin, being a zwitterion, diffuses across the host cell membrane and undergoes redox reactions by accepting electrons from NADPH which it subsequently donates to molecular oxygen to form primarily O 2 - and subsequently H 2 O 2 through dismutation ( The results detailed in Figure 5 provide a means to explain the pyocyanin/GPx/GRed/GSH interplay: that is, pyocyanin increases the level of ROS, and this leads to enhanced GPx activity in A549 cells because GPx catalyzes the reaction through which GSH neutralizes H 2 O 2

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