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The starting reference models for each dataset were either the previously determined cryo-EM structure of PV3-SC8 from a plant expression system 18 (EMDB accession code EMD-3747) low-pass filtered to 60 to avoid bias for PV3-SC8 GPP3+GSH and PV3-SC8 pleconaril+GSH or for wt PV2-CP17 a 50 low-pass filtered model was generated from a prior reconstruction of a subset of the data

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