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To examine whether sulfonylation of the cysteine residues affects the enzyme activity of OsDHAR, we grew Escherichia coli cells harbouring single genes [ OsDHAR (WT), OsDHAR (C20A) and ScSRX ] and dual genes [ OsDHAR (WT) + ScSRX and OsDHAR (C20A) + ScSRX ]

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Due to the existence of the conjugated system of C = C bonds and carbonyl groups, 4-HNE can provide partial positive charge to the carbon at position three and become efficient electrophiles, allowing it to react with essential biological molecules, including proteins and DNA (107109)