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Endpoints As this is an exploratory trial, we will set three primary endpoints: (1) resolution rate from the baseline of NASH (defined by the NASH CRN as no more than mild residual inflammatory cells [score of 0 or 1] and no hepatocyte ballooning [score of 0]) without the worsening of liver fibrosis, with worsening defined as an increase of one stage or more on the Kleiner fibrosis classification scale [34]) after 52 weeks of intervention

Cells from a 50 mL overnight culture in LB medium were harvested by centrifugation (4,000 g, 10 min) and resuspended in 20 mL MOPS minimal medium (Neidhardt et al., 1974)

Data use agreements and institutional IRB will be required

The slowed gastric emptying that reduces appetite also slows colonic motility