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These intestinal sections were flushed with ice-cold phosphate-buffered saline (PBS) to remove fecal matter and placed in 1- to 2-cm pieces into 15 mL sterile tubes with 5 mL dissociation buffer (PBS, 10 mM EDTA) for incubation at 37C in a shaker at 250 rpm for 20 minutes

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Dose-by-dose weight loss in Phase 2 (NEJM) Phase 2 NEJM weight loss by dose, 24 and 48 weeks Dose 1 mg 24 weeks -7.2% 48 weeks -8.7% Dose 4 mg 24 weeks -12.9% 48 weeks -17.5% Dose 8 mg 24 weeks -15.7% 48 weeks -22.8% Dose 12 mg 24 weeks -17.5% 48 weeks -24.2% Dose Placebo 24 weeks -1.6% 48 weeks -2.1% Source: Jastreboff et al., NEJM 2023

Retatrutide for Weight Loss Retatrutide is still being studied, but the numbers are eye-catching