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The further steps included incubation of specimens in 2% osmium tetroxide (Sigma-Aldrich) for 2 h at room temperature and dehydration in a series of ethanol solutions (Linegal Chemicals, Poland), starting from 10 min twice in 50 and 70%, followed by 5 min twice in 95% and 1 min twice in 100% ethanol solution

5.3 Impact on microbiome The intricate interplay between immune system modulation and microbial fluctuations across the body has recently gained considerable attention in various livestock species