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4) Tolerance: Effective is pointless if it irritates and forces me to stop

Participants were recruited from England, Wales, Scotland, Northern Ireland, and the Republic of Ireland

Samples were then washed with ice-cold PBS and incubated overnight with 10% sucrose in PBS followed by 20% sucrose and 10% glycerol in PBS

Peer review Peer review information Nature Nanotechnology thanks Steven Griffiths, Jiwei Zhai and the other, anonymous, reviewer(s) for their contribution to the peer review of this work