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The results showed that carotenoid significantly improved the serum concentrations of lutein, zeaxanthin, meso-zeaxanthin, and MP, and there was a significant difference in the dietary intake of lutein/zeaxanthin between the two groups

Luciferase reporter assay We co-transfected 0.5 mg of pGL4.10 vector expressing CPT1A -BS1 or CPT1A -BS2 (or the indicated mutant), along with 0.5 mg of pRL-TK plasmid (as a reference) and 50 ng of Renilla luciferase reporter into H1299 cells using Lipofectamine 2000

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