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Cells were then washed three times with ice-cold PBS and incubated for 1 h at 4 C with 1 mM bis(sulfosuccinimidyl) suberate (BS3, non-cell permeable cross-linker, Thermo Scientific) in PBS

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Carnosine An amino acid and potent antioxidant that protects skin firmness and elasticity, while reducing redness and protecting against oxidative stress

The finding of Ileibacterium as the most abundant cecal microbiome genus in every experimental group is relatively novel and may reflect experimental factors such as diet, housing conditions, and mouse strain