After 48 h, 160 l of expression medium (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-galactose (Panreac, A1131), 67 mM potassium phosphate monobasic buffer pH 6.0 (Thermo Scientific, 424200250), 25 g l 1 ethanol (Scharlau, ET00062500), 2 mM copper sulfate (Merck, C7631) and 25 g ml 1 chloramphenicol (Merck, C0378)) was added to each well
FIGURE 1 2.1 The main causes of IE 2.1.1 Abnormal proliferation and differentiation of erythroid hematopoietic progenitor cells The differentiation and maturation of erythroid cells involves several stages
We used generalized linear regression models to evaluate associations with ZBI score and logistic regression to identify factors that were independently associated with high caregiver burden (ZBI 21)
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