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They metabolize through different pathways

After overnight incubation, membranes were washed 3 times with TBST, incubated with IRDye secondary antibodies (1 h at RT), washed again 3 times again with TBST, and visualized using the LI-COR Odyssey FC 2800 imager

It is important to note that without the delineation of the MPIs, one would have concluded that especially proinflammatory cytokine genes are the dominant forces in this network, whereas, in fact, the IO&NS subnetwork is more dominated by redox genes, NF-B and IL-10