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Overcoming multidrug resistance by knockout of ABCB1 gene using CRISPR/Cas9 system in SW620/Ad300 colorectal cancer cells

LY294002 and SP600125 were from EMD (Gibbstown, NJ, USA) Western blot analysis The antibodies used for western blotting included those against stress-activated protein kinase (SAPK)/JNK, phospho-SAPK/JNK (Thr183/Tyr185), caspase-7, caspase-9, phospho-Bcl-2 (Ser70), and poly(ADP-ribose) polymerase (PARP) (Cell Signaling Technology, Danvers, MA, USA), cytochrome c and -actin (Sigma, St Louis, MO, USA), cytochrome oxidase subunit IV (Cox IV

The finding that acute injection of semaglutide reduces alcohol intake in male rats ( Similar to the results obtained with acute injection, repeated liraglutide treatment (six injections over 2 weeks) of alcohol-experienced male rats has been shown to decrease both alcohol consumption and preference for alcohol (Vallof et al., 2015)