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Introduction Acetaminophen ( N -acetyl- p -aminophenol, APAP, or paracetamol, PARA) is widely used for its analgesic and antipyretic properties in many over-the-counter formulations in both adults and children [1,2]

Briefly, 10 L of each sample (containing 2 mg/mL total protein) was loaded onto 5% staking and 8% native polyacrylamide gel bathed in 1 Tris-glycine buffer (pH 8.3) and the proteins separated at constant current (120 V) at 4C for 3 hours

this discrepancy is reasonable considering the yield of HGF after a wash-out step that often causes trapping/stacking of proteins on ultra-filtration membranes

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