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Some studies suggest that the pro-inflammatory cytokines IL-1 and TNF- also suppress the expression of NTCP, and impaired function of NTCP affect BA transport

[00:38:28] Michael: Well, it seems to me like even functional doctors here, I have a limited experience with this, but I have all functional doctors

Wash with PBS one more time and add 500 l of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice