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A standard curve was created using serial dilutions of plasmid DNA containing the target mtDNA sequence, and amplification efficiency was validated with a correlation coefficient above 0.98

Some bacteria also produce methylcobalamin and in the environment, methylcobalamin serves as a biomethylation compound of certain heavy metals

Additionally, ingestion of non-ethanol alcohols, such as methanol or isopropanol, found in products like antifreeze or certain solvents, causes severe poisoning

These compounds should not be considered interchangeable alternatives they belong to different categories of scientific maturity