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Allow vials to reach room temperature for 15 to 30 minutes before opening to prevent condensation Disinfect rubber stoppers with alcohol swab and allow complete evaporation Draw the calculated volume of bacteriostatic water into syringe Angle needle against vial wall and slowly inject water down the side, allowing it to gently slide onto powder Never squirt water directly onto lyophilized powder as this damages peptide structure Gently swirl or roll vial between palms to dissolve

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However, during prolonged storage, folate contents gradually dropped, reaching 25% and 38% losses in the bread leavened with bakers yeast and in the bread fermented with sourdough, resp., after 16 weeks, likely due to air oxidation