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This research was supported by National Demonstration Pilot Project for the Inheritance and Development of Traditional Chinese MedicineConstruction project between Guangzhou University of Chinese Medicine and Zhongshan Hospital of Traditional Chinese Medicine (No

The membrane was incubated in blocking solution (5% (w/v) skim milk powder (LP0031, Oxoid), 0.1% (v/v) Tween20 (9,005645, Ampresco), 1x PBS (137 mM NaCl (BP358-212, Fisher), 0.027 mM KCl (7447-40-7, Anachemia), 10 mM Na 2 HPO 4 (SDB0487, Biobasic), and 2 mM KH 2 PO 4FF ) for 1 h, shaking, at room temperature

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Polyphenol oxidase (PPO) is identified as the primary factor influencing postharvest browning in Agaricus bisporus (49)