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Its levels decline after age 60, and lower levels are linked to faster cognitive decline, while higher levels are associated with reduced risks of heart disease and overall mortality, suggesting its importance for healthy aging and disease prevention

Before the disease progresses to liver and brain (WD) or lung (inflammation), the excess copper can be limited by Cu(II) reduction, Zn(II) addition and administration of Cu(II) chelating ligands such as tetrathiomolybdate (TM), triethylene tetramine (Trientine, TETA, Trien) or D-penicillamine (D-pen) [119, 207] for limiting excess copper

The other set of samples was kept frozen immediately in liquid nitrogen at 80 C until the processing for RT-qPCR analysis