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Western blot analysis After washing with ice-cold PBS, cultured cells were lysed with buffer (50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1% Nonidet P-40) containing protease inhibitors (Roche Diagnostics, Basel, Switzerland)

241 Low-dose aspirin mitigates platelet hyperreactivity linked to high TMAO and may decrease TMAOs dependence on choline

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Keywords GHK peptide, sleep deprivation, learning impairment, neuroinflammation, nitrotyrosine Sleep deprivation is an increasing problem in modern society, with sleep duration among American adults decreasing over the past 25 years