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Sperm viability was quantified by mixing a 2L aliquot of sperm suspension with 5L of a 1:50 dilution of SYBR-14, followed by 2L of propidium iodide (Invitrogen, L-7011, Thermo Fisher Scientific, Melbourne, Australia)

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Etheresia Pretorius, orcid.org/0000-0002-9108-2384 Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

The effect of copper tripeptide and tretinoin on growth-factor production in a serum-free fibroblast model