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Influence of gestational salt restriction in fetal growth and in development of diseases in adulthood

platensis cells were high-pressure frozen using a Leica EM PACT2 instrument (Leica Microsystems, Buffalo Grove, IL), cryo-substituted for 80 h at 87 C in acetone containing 2% osmium tetroxide, and, after slowly warming to RT, embedded in Spurrs resin

276 In only one of the two sequences was there a significant increase in hematocrit

2 Materials and methods 2.1 Cell cultivation, differentiation, and treatment All experiments received approval from the Ethics Committee of the Third Hospital of Hebei Medical University