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363,364 These tumor-associated adipocytes release fatty acids into the microenvironment, which CSCs rapidly take up and metabolize through FAO

Group 4 (long-term diabetic cases) Four of 8 cases from this group showed an absence of insulin cells in 10 randomly selected islets per case

Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

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