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In brief, the thiol function of the cysteine methyl ester hydrochloride was first protected with a trityl (Trt) group and the selenoethyl arm which is Se -protected with a para -methoxybenzyl (PMB) group was then inserted by reductive amination reaction 50

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This is because it is a sensitive molecule that is not located in a controlled environment within the cell, as is the case with most enzymes, but functions outside the cell and is thus exposed to various disruptive influences

Glutathione synthase adds glycine to -GC resulting in glutathione formation