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This peptide, which he named exendin-4 , was approximately 53% identical in amino acid sequence to human GLP-1, but with one critical difference: while human GLP-1 is degraded by an enzyme called DPP-4 within minutes of being released, exendin-4 was resistant to that breakdown

It binds to the GLP-1R, stimulating insulin secretion, suppressing glucagon release, delaying gastric emptying, and promoting satiety

By noting exactly when and why a scheduled administration did not occur, investigators could better interpret the variability in outcomes

Blood (25 L) was collected from the retro-orbital sinus for up to 23 days