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In the process of sperm selection (e.g., density gradient centrifugation), PDEVs can protect the integrity of sperm membranes, and OFI-EVs from cactus fruits contain plant sphingolipid components that may reduce cryo-recovery damage by stabilizing sperm membrane structure (Valentino et al., 2024), Sesame-derived extracellular vesicles (SAEVs) may also improve fertilization rates by regulating calcium ion channels to promote sperm capacitation (Zhao et al., 2025)

doi: 10.1007/s00125-009-1347-2

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Amide bond-forming enzymes have found widespread interest as biocatalysts for the synthesis of pharmaceuticals because of the ubiquity of the amide functionality 29,30 (Extended Data Fig