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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)

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Morphological features were investigated by scanning electron microscopy (SEM, ZEISS EVO18), while energy-dispersive X-ray spectroscopy (EDX) was used to assess elemental composition

Details about this PCAC meeting can be found at this link