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T cell populations in children with autism spectrum disorder and co-morbid gastrointestinal symptoms

Cytokine levels were measured using enzyme-linked immunosorbent (ELISA) assay kits: Human 1L-10 ELISA Ready-SET-Go from Affymetrix (Cat# 88-7106) and Human TNF- Uncoated ELISA (Cat # 88-7346), Human IFN- Uncoated ELISA (Cat # 88-7316), and Human IL-6 Uncoated ELISA (Cat # 88-7066) from Invitrogen to quantity detection of cytokines

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It works by inhibiting the enzymes cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), which are involved in the production of prostaglandins compounds that promote inflammation and pain