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2.4.5 Validation of HPLC method The selectivity, sensitivity, standard curve, accuracy, precision, stability, and dilution reliability of the proposed approach were verified following the Guiding Principles for the Validation of Quantitative Analysis Methods for Biological Samples . To demonstrate the selectivity, chromatograms obtained from six batches of mixed blank chicken plasma, blank plasma spiked with DOX standard solutions and plasma samples after administration of DOX were compared

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