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Suspended cells were lysed with three freezing-thaw cycles in liquid nitrogen and subsequent ultra-sonication

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Always start retinol slowly (once or twice a week) and ensure your glutathione supplement is free from synthetic fillers that might trigger a reaction

Membranes were washed in TBST three times for 15 min each at room temperature on a shaker and incubated with polyclonal secondary antibody conjugated with horseradish peroxidase (HRP) in 5% BSA (Dako) for 1 h at room temperature