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The HBMECs were cultivated at a density of 5 10 4 cells/well on the upper insert for 3 d, and the media were replaced with those containing OG488-LPs and Lf-OG488-LPs

After staining, samples were washed in 100% methanol for 1 hour, followed by two washes of 2 hours each, and then left overnight at room temperature

This variant can occur de novo or can develop with any of the following histopathologic types: superficial spreading melanoma, LMM, nodular melanoma, or acral lentiginous melanoma

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