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cDNA was then used as template for RT-qPCR with a QuantStudio 3 Real-Time PCR system (Applied Biosystems) using PowerUP SYBR Green Master Mix (Applied Biosystems) and 500 nM of target gene specific forward and reverse primers, using the recommended fast cycling mode

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In this perspective, it is important to understand how CRISPR-Cas9 system can help in the crop improvement by harnessing the precision of genome editing of GSTs in different plant species