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Following CCK8 results showed that icFSP1 treatment abolished the ferroptosis protective effect of PPARa over-expression in N2A (Fig

For staining for mitochondria-rich cells (chloride cells), the cells were seeded on microscopy polymer coverslips (Ibidi, Grfelfing) and after 5 days the mitochondria were stained with MitoTracker Red CMXRos (Molecular Probes, Invitrogen

In contrast, a longer recovery period of 5 h post pre-cutting was required for the assessment of small molecules-activated gene expression levels

Consistent with previous reports (Nishiyama et al, 2010), ESCO1/2 KD reduced the SMC3ac levels as well as the chromatin-bound Sororin levels (Fig