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2.8 Determination of ROS levels The ROS were assessed via an ROS assay kit (Beyotime, China)

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Slight changes in pH have been reported to interfere with amino acid functional group ionization and impair hydrogen bonding, as a result protein folding is changed, leading to denaturation and cessation of enzymatic or other activities ( Variation of pH in the environment has a direct impact on the availability of Al, Fe, Mn, Cu, and plant growth