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Overall, the causes of Parkinsons disease are complex and not fully understood, but a combination of genetic and environmental factors is thought to contribute to the development of the disease
PLoS ONE 8:e55375 Collier TJ, Kanaan NM, Kordower JH (2017) Aging and Parkinsons disease: different sides of the same coin
The cells were then washed once in ice-cold PBS, trypsinized, pelleted, and then homogenized in mitochondrial isolation buffer (0.21 M mannitol, 0.07 M sucrose, 0.1 mM EDTA, 1 mM EGTA, 10 mM Tris HCl, 0.5% BSA, pH 7.4 using KOH)
Chen, H., Zhang, X., Liao, N., Ji, Y., Mi, L., Gan, Y., Su, Y., & Wen, F
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