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In VRK2-KO cells, most of cells displayed ER distribution of SLC7A11 regardless of monensin addition or removal, suggesting that VRK2 did not have an influence on Golgi-to-PM trafficking of SLC7A11 (Fig

NCTC1469 cells, a normal mouse hepatocyte cell line, were obtained from the American Type Culture Collection and were cultured in DMEM containing 10% (v/v) fetal horse serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

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Oral bioavailability studies in rats show systemic effects even when administered per os, suggesting meaningful absorption through the GI mucosa