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One unit of CAT was defined as the amount of protein required to convert 1 M of H2O2 to H 2 O and O 2 per minute ( 2.10 Glutathione peroxidase activity The GPx activity was monitored by determining the decrease in nicotinamide adenine dinucleotide phosphate (NADPH) analytical signal at 340 nm using a scanning spectrophotometer (AJX-6100PC, Micronal) for 3 min at 20C ( 2.11 Quantification of the redox state by reduced glutathione and oxidized gluthathione relation The GSH/GSSG ratio was assessed by measuring the levels of GSH and GSSG ( 2.12 Sulfhydryl group content Total protein quantification of the placental explants was conducted using the Bradford method, and a BSA solution (3 mg mL-1) was used as a standard for total protein determination

423,480 Gao et al

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