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After cross-linking treatment, the cells were washed with DEPC-treated PBS and collected in ice-cold, RNase-free IP150 lysis buffer (prepared using DEPC-treated water, consisting of 50 mM HEPES pH 7.5, 150 mM NaCl, 10% glycerol (G8190, Solarbio, Beijing, China), 0.5% Triton X-100 (T8200, Solarbio, Beijing, China), 1 mM EDTA, 1 mM DTT, 1 l RNase inhibitor, and protease inhibitor)

Genetic Factors and Glutathione Synthesis Capacity Several genetic variants influence glutathione production and antioxidant enzyme activity

This mechanism founds research on skin depigmentation and radiance, the field where glutathione is the most studied candidate

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