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As a result, the main melanogenesis regulator MITF is elevated after UVR, followed by an increase in other melanosomal proteins such as TYR, TRP-1 and TRP-2, leading to the stimulation of melanogenesis [47]

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Western blot and real-time PCR Real-time PCR was performed using a Roche LightCycler 480 detection system (Roche, Basel, Switzerland)