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AAV vectors are generally safe at therapeutic doses eight AAV-based gene therapies have been FDA-approved as of 2024 but at high doses, above roughly 10^14 viral genomes per kilogram, the literature documents immune reactions, liver toxicity, and in rare cases, genotoxicity
10.1016/j.steroids.2022.109055 70 MoreS
To disentangle the metabolic impact of estrogen-deficiency driven gut microbial changes from the systemic effects of estrogen deficiency itself, fecal samples were transplanted from ovariectomized mice into male germ-free recipient gonadal-intact mice (109)
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