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Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change ( TM DE kit is based primarily on human activin A and Wnt3a ( Hepatoblast specification For small molecule hepatoblast specification, DE cells were washed with DPBS / and knockout DMEM (Thermo Fischer Scientific) and sequentially cultured with hepatoblast specification medium without passaging

23 Early lifestyle interventions, such as recommendations for optimizing diet, should be offered to all pregnant women with PCOS to mitigate risk factors

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