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No heterogeneity was presented in the included studies, (I 2 = 0%, P = 0.65), and so the fixed effects model was used (Fig

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Similarly, recombinant PDI was diluted to 0.3 mg ml 1 in 100 mM sodium phosphate buffer (pH 7.0) containing 0.3 mM 1,8 ANS and thermal-shift assay was performed as described above