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Enzyme Activities For enzyme activities, cells and mouse tissues (duodenum, liver, spleen) were homogenized with a TissueLyser II (Qiagen, Hilden, Germany) in Tris buffer (100 mM Tris (Carl Roth), 300 mM KCl (Applichem, Darmstadt, Germany), pH 7.6 with 0.1% ( v/v ) Triton X-100 (Serva, Heidelberg, Germany), and 0.1% ( v/v ) protease inhibitor (Merck/Millipore, Burlington, MA, USA)) two times for 60 s at 30 Hz

The KFF survey findings are based on responses that were collected from a nationally representative sample of nearly 1,500 adults during the last week of April

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Other JAK inhibitors that have shown benefits in placebo-controlled trials in alopecia areata patients include deuruxolitinib (Leqselvi, approved by the FDA in 2024) and brepocitinib (King 2021