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Daytime fatigue paired with nighttime restlessness can occur when eating patterns, activity levels, or hydration change at the same time

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When GLP-1 signaling reaches those areas, research suggests the intensity of that reward prediction may decrease

Tissues were blocked in CAS block (Thermo Fisher Scientific) and incubated overnight with primary antibodies diluted in CAS block (Table 1): Primary antibodies for immunofluorescence Slides were washed 3 times in PBST, incubated overnight with secondary antibodies, and diluted in PBS/BSA 1% (Table 2): Secondary antibodies for immunofluorescence Slides were then washed once in PBS and closed with cover glass using ImmuGio mounting medium (DiaSorin, 2505-5)