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Site-directed mutagenesis Site-directed mutagenesis of the GST clone was performed using a QuikChange mutagenesis kit (Stratagene) according to the manufacturers protocol
It comes with Methionine, Inositol, Choline, Leucine, L-Carnitine, Vitamin C, B1, B2, B3, B5, B6, and B12
Step 6: Remove the syringe
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