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Reconstitution of TRAF3 in human multiple myeloma cell lines inhibited ChoK expression, suppressed the Kennedy pathway, and induced apoptosis, underscoring the role of elevated choline metabolism in sustaining the phenotype of TRAF3-deficient malignant B cells

Ray Dorsey E, Elbaz A, Nichols E, Abd-Allah F, Abdelalim A, Adsuar JC, et al

Reconstitution steps: Allow vial to reach room temperature before opening Wipe rubber stopper with an alcohol swab and allow to dry Insert needle bevel-up at a 45 angle through the stopper Inject bacteriostatic water slowly along the glass wall, do not aim at the lyophilised cake Gently swirl until fully dissolved, do not vortex, shake, or sonicate Inspect for clarity and absence of particles before use Storage: Lyophilised vials (store at 20C, protected from light)

Human Erythrocyte Acetylcholinesterase in Health and Disease