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Solute carrier family 39 member 6 gene promotes aggressiveness of esophageal carcinoma cells by increasing intracellular levels of zinc, activating phosphatidylinositol 3-kinase signaling, and up-regulating genes that regulate metastasis

To construct the pPapi-SLC25 library, SpCas9 and SaCas9 guides were designed to target 53 SLC25 carriers, seven additional SLC proteins present in MitoCarta 2.0 16 , BCL2L1, MCL1 (the two paralogous anti-apoptotic proteins) and EEF2 (elongation factor 2, essential for protein translation) as positive controls, 8 non-cutting controls and 19 cutting controls that target olfactory receptors (ORs) as negative controls

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Mol Psychiatry 2015