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Regarding the mean intensity of Flag-ERK5 in the nucleus, both EGF stimulation and AX15836 treatment induced Flag-ERK5 translocation into the nucleus, whereas RNK06138 treatment decreased the intensity of nuclear staining (Fig
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Molecules 24 (8), 1582
However, recombinant proteins used were often devoid of the Fe-S cluster which is problematic because four cysteine residues now become possibly reactive and blur the conclusions
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